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Invitrogen
PIR-A/B Monoclonal Antibody (10-1-PIR), APC, eBioscience™
FIGURE: 1 / 4
PIR-A/B Antibody (17-3101-80) in Flow
Staining of C57BL/6 splenocytes with Anti-Mouse CD19 FITC (Product # 11-0193-82) and 0.125 µg of Rat IgG2b kappa Isotype Control APC (Product # 17-4031-82) (left) or 0.125 µg of Anti-Mouse PIR-A/B APC (right). Total viable cells were used for analysis.
Product Details
17-3101-80
Applications
Tested Dilution
Publications
Product Specifications
Published species
Not Applicable
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
10-1-PIR
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_1944407
Product Specific Information
Description: This 10-1-PIR monoclonal antibody reacts with mouse paired Ig-like receptors of activating (PIR)-A and -B. These cell surface glycoproteins, which contain six extracellular Ig-like domains with distinct transmembrane and cytoplasmic regions, are expressed together on B lymphocytes, dendritic cells, macrophages, granulocytes, platelets, and mast cells. However, expression of PIR-A/B has not been observed on T cells, NK cells, and erythrocytes. The PIR-A receptor interacts with signaling molecules containing immunoreceptor tyrosine-based activation motifs (ITAMs) which lead to its activating function. In contrast, the PIR-B receptor associates with proteins possessing immunoreceptor tyrosine-based inhibitory motifs (ITIMs); therefore, this receptor has been shown to have an inhibitory function. PIRs bind MHC class I to modulate cell signaling and homeostasis of the immune system. Moreover, PIR-B knockout mice have been shown to exhibit susceptibility to Salmonella infection.
Crossblocking studies indicate that 10-1-PIR recognizes a different epitope from 6C1 (Product # 46-5978).
Applications Reported: This 10-1-PIR antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 10-1-PIR antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Paired Ig-like receptors of activating (PIR)-A and -B are expressed on B lymphocytes, macrophages, dendritic cells, platelets, granulocytes, and mast cells, but not on T cells, NK cells, and erythrocytes. These cell surface glycoproteins contain six extracellular Ig-like domains with distinct cytoplasmic and transmembrane regions. The PIR-A receptor activates through signaling molecules containing immunoreceptor tyrosine-based activation motifs. In contrast, the PIR-B receptor inhibits through proteins possessing immunoreceptor tyrosine-based inhibitory motifs. PIRs bind MHC class I to modulate immune system homeostasis and cell signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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